Service
Mammalian Cell Transient Transfection
Mammalian transient expression systems are very widely used in many fields for applications such as: early stages development of biopharmaceutical protein drugs and vaccines, in vivo toxicity experiments, diagnostic testing, as well as many others. Transient expression systems offer the advantage of providing a host of proteins chaperones involved in protein folding while your protein is being expressed in these cells. With CHO and HEK293 suspension cells serving as the expression hosts for the JSBio Mammalian Cell Transient Transfection Platform, our customers can expect complete, stable, and efficient production of protein with ranging from milligram to as much as a gram of protein.
Platform Advantages
More Efficient
The transfection process is streamlined and efficient, with protein expression detected as early as 2 - 3 days after transfection.
More Capable
Using our proprietary cell culture media formulations in combination with transfection reagents and specialized transfection equipment, JSBio maximizes the performance advantages of this platform.
More Cost-effective
By utilizing our platform and services, the customer saves the cost associated with expensive equipment purchases, consumables, plasmid preparation and cell culturing time of personnel, etc. Outsourcing for your protein production needs saves the production cost of unit protein yield dramatically.
Platform of mammalian cell transient transfection systems
CHO Transient Transfection System | HEK293 Transient Transfection System | HEK293 Transient Transfection System | |
Cell | CHO cell | 293 cell | 293 cell |
Growth Medium | CD CHO Transfection Media | CD 293 Medium | HEK293 SFM 616 |
Transfection Reagent | Electroporation Buffer | Transfection Helper | Electroporation Buffer |
Growth Medium post Transfection | CD CHO Transfection Media | CD 293 Transfection Medium | HEK293 SFM 616 |
Expression Medium post Transfection | CD CHO Transfection Feed | HEK293 Transfection Feed | HEK293 Transfection Feed |
Expression Enhancer | Feed B (100×) | Feed B (100×) | Feed B (100×) |
Suggested Transfection Cell Density | 5.0 × 106 cells/mL | 3.0 × 106 cells/mL | 3.0 - 6.0 × 106 cells/mL |
Transfection Method | Electroporation | Chemical transfection | Electroporation |
Medium Volume | 40 - 2000 mL | 40 - 2000 mL | 40 - 2000 mL |
Titer | ＞3.0 g/L | 1.0 g/L | 1.0 g/L |
Service Content
Description | Experimental content | Delivery |
Vector Construction | Option 1：The client provides an amino acid sequence or gene sequence, then JSBio clones it into an expression plasmid Option 2：The client provides an established vector. | Sequencing report Lab report |
Transient Transfection | Host cell without vector proliferation and passaging →Transient Transfection →Fed-batch expression→ product harvest and test (Choose from HPLC, ELISA, and Fortebio） | Production and yield report Supernatant |
Protein purification (optional) | Protein A once-step purification (proceed to SEC-HPLC, SDS-PAGE or WB based on need） | Protein purification, Purification experiments, and protein Detection report |
Service Flow Chart
Description of client requests and confirmation of study plan details
Contract signining
Transient expression of protein
Isolation and purification of product
Delivery of product
Follow-up tech support
Ordering Information
CHO Cell Transient Transfection Media
Product Name | Catalog No. | Package Size | Application |
CD CHO Transfection Media | 88035-603 | 5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized | CHO passage and proliferation |
CD CHO Transfection Feed | 99101-604 | 1 L/ 2 L/ 5 L/ 10 L/ 50 L/ Customized | CHO protein expression post transfection |
Feed B (100×) | 99035-16018 | 100 mL/ 250 mL/ Customized | Further increase protein yield |
HEK293 Transient Transfection Media (Chemical Transfection）
Product Name | Catalog No. | Package Size | Application |
CD 293 Medium | 11201-706 | 2 L/ 5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized | HEK293 passage and proliferation |
Transfection Helper | 99124-708 | 100 mL/ 250 mL/ Customized | Plasmid Dilatant，Improve transfection efficiency |
CD 293 Transfection Medium | 99102-617 | 2 L/ 5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized | HEK293 Transient transfection and protein expression |
HEK293 Transfection Feed | 99012-617 | 1 L/ 2 L/ 5 L/ 10 L/ 50 L/ Customized | HEK293 protein or LV expression post transfection |
Feed B (100×) | 99035-16018 | 100 mL/ 250 mL/ Customized | Further increase protein or LV yield |
HEK293 Transient Transfection Media (Electroporation）
Product Name | Catalog No. | Package Size | Application |
HEK293 SFM 616 | 10808-616 | 2 L/5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized | HEK293 passage and proliferation |
Electroporation Buffer | 99108-21005 | 100 mL/ 250 mL/ Customized | Transfection Reagent for Improving transfection efficiency |
HEK293 Transfection Feed | 99102-617 | 1 L/ 2 L/ 5 L/ 10 L/ 50 L/ Customized | HEK293 protein expression post-transfection |
Feed B (100×) | 99035-16018 | 100 mL/ 250 mL/ Customized | Further enhance protein yield |
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