Transient expression

• Basic Research

• Druggability Evaluation
  

Synthesis  of a gene and cloning into a vector to provide expression plasmids containing your gene of interest, expression reports, and purified products（optional）

CHO

Electroporation

HEK293

Chemical-based transfection

HEK293

Electroporation

Stable expression

• Long-term studies of gene expression or protein function

•  Non-GMP production of recombinant protein for IND filing

Synthesis  of a gene of interest and cloning into an expression vector for providing a stable cell line ,documention of all studies performed, and purified products(optional)

CHO

Electroporation

Vector Construction

Option 1: The customer provides the target protein sequence, and the JSBio platform uses the self-developed high expression vector to construct the plasmid

Option 2: The customer provides the own established vector

Sequencing Report;

Study Report

Transient Transfection

Parental cell passaging and expansion → transient transfection → Fed-batch production → product harvest and detection（HPLC, ELISA, Fortebio，or SDS-PAGE, WB per request）

Detection Report;

Production Report;

Supernatant 

Protein Purification

（optiomnal）

Protein A one step purification

Protein Purification;

Purification study and detection Reports

Delivery time: Samples shipped within 2 - 3 weeks after the contract is signed

Vector Construction

Option1: The customer provides the target protein sequence, and the JSBio platform uses the self-developed high expression vector to construct the plasmid

Option2: The customer provides their own established vector

Sequencing Report

Study Report

Stable Transfection

Transfection → Pool expression yield determination

 High-expression Minipool screening Report

Mimipool Screening

Selection of 5 - 8 high-expression Minipool

Single Clone Selection

Selection of 3 - 5 high-yield cell lines → Stability detection → Confirm one high-yield cell clone

3 high-yield cell clones（Optional）;

Single Clone selection report;

Stability Detection Report;

One high-yield Cell clone delivered

Process Scale-up

（Optional)

Protein;

PD Report

Delivery time: Shipping is 4 - 6 months post contract signing