Service
Non-GMP Production of Recombinant Protein

High quality recombinant protein production is key for basic science research and pre-clinical drug development studies. Our team members at JSBio are expects in new and advancing techniques in the industry for cell line and cell culture medium development. We have established a non-GMP recombinant protein production platform for both transient and stable transfection in mammalian cells. Clients may choose between CHO or HEK293 cells to serve as the host for our transient transfection platform which yields milligram to even gram-level production of your recombinant protein on a time scale of 2 - 3 weeks. Clients may also choose our stable cell transfection platform which utilizes CHO cells to support large-scale recombinant protein production. Both platforms provide protein yields and production times that are highly competitive within this industry.


Platform Advantages
High efficiency and high quality services

We have established service agreements with multiple shipping providers, nationally and internationally.  We also have a logistics team on-site who are very responsive to client’s questions and concerns regarding shipping and handling of their product.


Expedited delivery time

Please allow 2 - 3 weeks for products made using transient transfection and 4 - 6 months for products generated using stably expressing cells. For client’s using our integrated cell culture medium and process development platform, time frames will vary based on the specific needs of your project.


Better value for your expense

JSBio uses our proprietary, high quality media formulations to optimize protein yield which significantly reduce cost for the client.


Service Content Selection Guide

Expression methods

Applications 

Prorerty Description

Cells

Transfection Methods

 Transient expression

  • Basic Research

  • Druggability Evaluation

 

Synthesis  of a gene and cloning into a vector to provide expression plasmids containing your gene of interest, expression reports, and purified products(optional)


CHO

Electroporation

HEK293

Chemical-based transfection

HEK293

Electroporation

Stable expression

  • Long-term studies of gene expression or protein function

  •  Non-GMP production of recombinant protein for IND filing


Synthesis  of a gene of interest and cloning into an expression vector for providing a stable cell line ,documention of all studies performed, and purified products(optional)


CHO

Electroporation


CHO/HEK293 Transient Transfection Platform

The transient expression platform utilizing CHO or HEK293 cells as a host can quickly provide customers with purified recombinant proteins on the scale of milligram (mg) to gram (g) amounts to meet your R&D needs.

Lab Procedure

Experimental Content

Delivery

 

Vector Construction

Option 1: The customer provides the target protein sequence, and the JSBio platform uses the self-developed high expression vector to construct the plasmid

Option 2: The customer provides the own established vector

Sequencing Report;

Study Report


 

Transient Transfection

Parental cell passaging and expansion → transient transfection → Fed-batch production → product harvest and detection(HPLC, ELISA, Fortebio,or SDS-PAGE, WB per request)

Detection Report;

Production Report;

Supernatant 


Protein Purification

(optiomnal)


Protein A one step purification

Protein Purification;

Purification study and detection Reports

Delivery time: Samples shipped within 2 - 3 weeks after the contract is signed


Service Flow Chart
  1. Exchange of client requests and plan confirmation

  2. Contract Signing

  3. Transient expression of protein

  4. Product harvest and purification(optional)

  5. Delivery of product

  6. Follow-up Tech Support

Related Products
CHO Transient Transfection Cell Culture Media

Product Name

Catalog No.

Package Size

Application

CD CHO Transfection Media

88035-603

2 L/5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized

CHO cell passaging and expansion

CD CHO Transfection Feed

99101-604

1 L/2 L/5 L/10 L/50 L/ Customized

CHO cell protein expression post transfection

Feed B(100×)

99035-16018

100 mL/ 250 mL/ Customized

Further improve protein yield


HEK293 Transient Transfection Cell Culture Media(Electroporation)

Product Name

Catalog No.

Package Size

Application

HEK293 SFM 616

10808-616

2 L/ 5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized

HEK293 cell passaging and expansion

Elextroporation Buffer

99108-21005

100 mL/ 250 mL/ Customized

Transfection reagent,to improve transfection efficiency

HEK293 Transfetion Feed

99102-617

1 L/ 2 L/ 5 L/ 10 L/ 50 L/ Customized

HEK293 cell protein expression post transfection

Feed B(100×)

99035-16018

100 mL/ 250 mL/ Customized

Further improve protein yield


HEK293 Transient Transfection Cell Culture Media(Chemical-based Transfection)

Product Name

Catalog No.

Package Size

Application

CD 293 Medium

11201-706

2 L/5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized

HEK293 cell passaging and expansion

Transfection Helper

99124-708

100 mL/250 mL/ Customized

Plasmid diluent, to improve transfection efficiency

  CD 293 Transfection Medium

11202-707

2 L/5 L/ 10 L/ 50 L/ 100 L/ 500 L/ Customized

HEK293 Transient transfection and protein expression

HEK293 Teansfection Feed

99012-617

1 L/ 2 L/ 5 L/ 10 L/ 50 L/ Customized

HEK293 Protein or LV viral expression post transfection

Feed B(100×)

99035-16018

100 mL/ 250 mL/ Customized

Further increase protein or LV viral yield


Stable Expression in CHO Cells

Customers will provide a protein sequence of interest to JSBio to complete the agreed upon services for, including one or multiple of the following: molecular cloning to generate the expression plasmid containing your gene of interest, cell line development of high-yielding single cell or pooled clones, process development, and scale-up.

Study procedure 

Experiential content

Delivery

Vector Construction

Option1: The customer provides the target protein sequence, and the JSBio platform uses the self-developed high expression vector to construct the plasmid

Option2: The customer provides their own established vector


Sequencing Report

Study Report


Stable Transfection

Transfection → Pool expression yield determination

 

 High-expression Minipool screening Report


Mimipool Screening

Selection of 5 - 8 high-expression Minipool

Single Clone Selection

Selection of 3 - 5 high-yield cell lines → Stability detection → Confirm one high-yield cell clone

3 high-yield cell clones(Optional);

Single Clone selection report;

Stability Detection Report;

One high-yield Cell clone delivered


Process Scale-up

(Optional)


2 - 15 L bioreactor process development → gradual scale up → Tech transfer

Protein;

PD Report

CMC Report

Delivery time: Shipping is 4 - 6 months post contract signing


Service Flow Chart
  1. Description of client requests and confirmation of project details

  2. Contract Signing

  3. Development and selection for a single cell clone with high product yield

  4. Validation of Process Development in bioreactors

  5. Tech Teansfer

  6. Delivery of product

  7. Follow-up tech support


Related Products
Fed-batch Process

Celkey® CHO Cell Cuture Media

Product

Catalog No.

Component Description

L-Gln

HT

Gluc

Pluronic F-68

(PF-68)

NaHCO3

CD CHO 011

88011-317

X

X

8.0 g/L

CD CHO 012

88012-318

X

X

8.0 g/L

CD CHO 050

88050-563

X

X

8.0 g/L

 

Celsource®  CHO  Cell Feed Media

Product

Catalog No.

Component Description

L-Gln

HT

Gluc

Pluronic F-68

(PF-68)

NaHCO3

CD Feed 002

99014-302

X

X

X

CD Feed 008

&

CD Feed 009

99084-499

&

99085-483

X

X

X


Perfusion Process

Celkey® CHO  Cell Perfusion Media

Product

Catalog No.

Component Description

L-Gln

HT

Gluc

Pluronic F-68(PF-68)

NaHCO3

CD CHO 014

88014-319

X

X

0.8g/L

CD CHO 024

80024-320

X

X

8.0g/L

CD CHO 051

88051-539

X

X

8.0g/L